Culture medium recipe
Vol.
×
Main sol. 778a #
ml
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-9,10-anthraquinone-2,6-disulfonate
(sc-397516A) |
8.25 | g | 8.25 | - | |
KH2PO4 | 0.33 | g | 0.33 | 2.425 | |
NH4Cl | 0.33 | g | 0.33 | 6.169 | |
KCl | 0.33 | g | 0.33 | 4.427 | |
MgCl2 x 6 H2O | 0.33 | g | 0.33 | 1.623 | |
Trace element solution SL-10 | 1.00 | ml | - | - | |
Selenite-tungstate solution | 1.00 | ml | - | - | |
NiCl2 x 6 H2O
(0.1% w/v) |
0.20 | ml | 2.0e-4 | 0.002 | |
Yeast extract | 1.00 | g | 1 | - | |
NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
CaCl2 x 2 H2O | 0.33 | g | 0.33 | 2.245 | |
Wolin's vitamin solution | 10.00 | ml | - | - | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except bicarbonate, calcium chloride and vitamins), boil medium for some minutes to dissolve the anthraquinone, then cool under 80% H2 and 20% CO2 gas atmosphere to room temperature. Add solid bicarbonate and adjust pH to 7.2, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary. |
Solution occurs in 262
other media (first defined in Medium 320).
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
Distilled water | 990.0 | ml | - | - | |
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. |
Solution occurs in 173
other media (first defined in Medium 385).
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaOH | 0.5 | g | 0.5 | 12.501 |
Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
Distilled water | 1000.0 | ml | - | - |
Solution occurs in 351
other media (first defined in Medium 141).
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | - |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
No comments for this medium.