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Main sol. 791

Internal Solution-ID: S1597

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na glutamate 0.5 g/l 0.5 2.957
Sodium succinate 0.5 g/l 0.5 3.085
Sodium acetate 0.5 g/l 0.5 6.095
Yeast extract 0.5 g/l 0.5 -
Casamino acids 0.5 g/l 0.5 -
(NH4)2SO4 0.5 g/l 0.5 3.784
Sodium thiosulphate 0.5 g/l 0.5 3.162
Basal salts solution 5.0 ml - -
Phosphate solution 5.0 ml - -
Vitamin solution 1.0 ml - -
Distilled water 989.0 ml - -
1 Final pH 7.5
2 Add the vitamin solution, aseptically to the cooled, autoclaved medium.
791 PORPHYROBACTER TEPIDARIUS MEDIUM