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Main sol. 793

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Internal Solution-ID: S1620

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.33 g 0.328 2.41
NH4Cl 0.33 g 0.328 6.133
KCl 0.33 g 0.328 4.4
MgCl2 x 6 H2O 0.33 g 0.328 1.614
CaCl2 x 2 H2O 0.33 g 0.328 2.231
Yeast extract 0.50 g 0.497 -
Glycerol
(87%) 		3.00 ml 2.594 28.173
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 5.00 g 4.97 46.894
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.497 2.069
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, vitamins, and sulfide, then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dissolve carbonate and adjust pH to 6.7, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration.
793 MOORELLA GLYCERINI MEDIUM