Main sol. 807
Internal Solution-ID: S1638
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 17.00 | g | 17 | 68.972 | |
| NaCl | 100.00 | g | 100 | 1711.16 | |
| Solution A | 50.00 | ml | - | - | |
| Solution B | 50.00 | ml | - | - | |
| Trace element solution SL-10 | 10.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| Yeast extract | 10.00 | g | 10 | - | |
| Trypticase | 10.00 | g | 10 | - | |
| Glucose | 5.00 | g | 5 | 27.753 | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.041 | |
| NaHCO3 | 4.10 | g | 4.1 | 48.806 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Distilled water | 890.00 | ml | - | - | |
| 1 pH 7.0 | |||||
| 2 Prepare the medium without yeast extract, glucose, NaHCO3, cysteine hydrochloride and Na2S x 9 H2O. Boil and then cool under a gas atmosphere of N2:CO2 (80:20 v/v) and add the NaHCO3 and cysteine hydrochloride. Autoclave the medium and add from sterile stock solutions yeast extract, trypticase, glucose, and Na2S x 9 H2O. The final pH should be 7.0. | |||||
Media with this solution
| 807 | HALOANAEROBIUM ALCALIPHILUM MEDIUM |