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Ferric EDTA solution

Internal Solution-ID: S1642

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 17.90 g 17.9 53.241
NaOH 3.28 g 3.28 82.006
FeSO4 x 7 H2O 13.70 g 13.7 49.278
Distilled water 1000.00 ml - -
1 1) Dissolve 17.9 g sodium EDTA and 3.28 g NaOH in 186 ml of distilled water.
2 2) Dissolve 13.7 g FeSO4 x 7 H2O in 364 ml of distilled ater, bubbling with air overnight, and store in a brown glass bottle.
3 3) Combine the two solutions and make up to 1 litre with distilled water.
808 SS1-MEDIUM