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Main sol. 824

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Internal Solution-ID: S1672

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-formate 2.72 g 2.709 39.836
KH2PO4 0.20 g 0.199 1.464
NaCl 0.50 g 0.498 8.522
KCl 1.00 g 0.996 13.36
MgSO4 x 7 H2O 0.50 g 0.498 2.021
CaCl2 x 2 H2O 0.15 g 0.149 1.016
NH4Cl 0.25 g 0.249 4.655
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Creatine 3.80 g 3.785 28.863
Yeast extract 2.00 g 1.992 -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.0e-4 0.002
Na2CO3 1.50 g 1.494 14.096
Wolin's vitamin solution (10x) 1.00 ml - -
Seven vitamins solution 1.00 ml - -
Na2S x 9 H2O 0.50 g 0.498 2.073
Distilled water 1000.00 ml - -
1 Dissolve ingredients except carbonate, vitamins and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.5 - 8.0.
824 TISSIERELLA CREATINOPHILA MEDIUM