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824. TISSIERELLA CREATINOPHILA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.72 g 2.688 39.522
2 0.20 g 0.198 1.452
3 0.50 g 0.494 8.454
4 1.00 g 0.988 13.255
5 0.50 g 0.494 2.005
6 0.15 g 0.148 1.008
7 0.25 g 0.247 4.618
8 Trace element solution SL-10 1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10 3.80 g 3.755 28.635
11 2.00 g 1.976 -
12
(0.1% w/v)
0.50 ml 4.9e-4 0.002
13 1.50 g 1.482 13.985
14 Vitamin solution 9.00 ml - -
15 Vitamin solution 1.00 ml - -
16 0.50 g 0.494 2.057
17 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients except carbonate, vitamins and sulfide. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.5 - 8.0.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.198 55408.3
Creatine 3.755 28.635
Sodium formate 2.688 39.522
Yeast extract 1.976 -
Na2CO3 1.482 13.985
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 2 strains

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