Main sol. 826
Internal Solution-ID: S1675
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.50 | g | 1.484 | 27.738 | |
| Na2HPO4 | 0.60 | g | 0.593 | 4.181 | |
| KCl | 0.10 | g | 0.099 | 1.327 | |
| Na-acetate | 0.82 | g | 0.811 | 9.887 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| NaHCO3 | 2.50 | g | 2.473 | 29.436 | |
| Na2-fumarate | 8.00 | g | 7.913 | 49.445 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, fumarate and vitamins), sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and equilibrate the medium with 80% N2 and 20% CO2 gas mixture to reach a pH of 6.8, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add fumarate and vitamins from anoxic stock solutions prepared under 100% N2 gas atmosphere and sterilized by filtration. Check medium pH and adjust to 6.8, if necessary. | |||||
Media with this solution
| 826 | GEOBACTER SULFURREDUCENS MEDIUM |