Main sol. 829d
Internal Solution-ID: S1684
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Sea Salt
(SIGMA) |
30.00 | g | 29.412 | - | |
NH4Cl | 0.30 | g | 0.294 | 5.499 | |
KH2PO4 | 0.35 | g | 0.343 | 2.521 | |
MES [2-(N-morpholino) ethane sulfonic acid] | 1.95 | g | 1.912 | 9.792 | |
Na-acetate | 0.16 | g | 0.157 | 1.912 | |
Modified Wolin's mineral solution | 10.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
Sulfur , powdered | 10.00 | g | 9.804 | 305.704 | |
Na2CO3 | 0.50 | g | 0.49 | 4.625 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
Na2S x 9 H2O | 0.50 | g | 0.49 | 2.041 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients (except sulfur, carbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.0 and dispense under 80% H2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials which contain already the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. Autoclave at a temperature of 110°C for 20 min! Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are sterilized by filtration. Adjust pH of the complete medium to 6.0 - 6.5, if necessary. | |||||
2 After inoculation add 1 bar overpressure of sterile 80% H2 and 20% CO2 gas mixture. |
829d | DESULFUROBACTERIUM INDICUM MEDIUM |