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Main sol. 1006

Internal Solution-ID: S17

Equipment needed: Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 7.80 g 7.769 50.072
2 4.00 g 3.984 -
3
(BD BBL)
1.00 g 0.996 -
4 0.29 g 0.289 1.658
5 0.29 g 0.289 2.123
6 0.48 g 0.478 3.618
7 0.48 g 0.478 8.18
8 0.10 g 0.1 0.404
9 64.00 mg 0.064 0.434
10
(0.1% w/v)
0.50 ml 5.0e-4 0.002
11 4.00 g 3.984 37.59
12 Volatile fatty acid mixture 3.10 ml - -
13 0.50 g 0.498 2.835
14 1000.00 ml 994.23 55188.1
Instructions:
  1. Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary.
1006 ALLISONELLA MEDIUM