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13. BACILLUS ACIDOCALDARIUS MEDIUM

Culture medium recipe

Vol.
×

M 13 #

ml
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 500 ml - -
2 Solution B 500 ml - -
Instructions:
  1. Sterilize solution A and B separately at 121°C for 15 min. Cool to 50°C and then combine.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 2 -
2 0.20 g 0.4 3.027
3 0.50 g 1 4.057
4 0.25 g 0.5 3.401
5 0.60 g 1.2 8.818
6 500.00 ml 998.207 55408.8
Instructions:
  1. Adjust pH to 3.0 - 4.0.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1 g 2 11.102
2 20 g 40 -
3 500 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 3.5

Associated strains

All strains for this medium: 8
Strains with modifications: 7

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar 20 -
Glucose 1 5.551
Yeast extract 1 -
KH2PO4 0.6 4.409
MgSO4 x 7 H2O 0.5 2.029
CaCl2 x 2 H2O 0.25 1.701
(NH4)2SO4 0.2 1.514

Cultivation metadata from 8 strains BacDive

halophily not 6 strains
halophily slightly 6 strains
halophily moderate 2 strains
pH 2 - 4 6 strains
pH 4 - 6 6 strains
phyla 1 8 strains
oxygen aerobe 6 strains
temperature thermophilic 8 strains

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