Co-factors solution
Internal Solution-ID: S1730
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Thiamine pyrophosphate | 2.50 | g | 2.5 | 5.426 | |
| Pyridoxal hydrochloride | 0.25 | g | 0.25 | 1.228 | |
| Pyridoxal phosphate | 0.25 | g | 0.25 | 1.012 | |
| Ca-folinate | 0.05 | g | 0.05 | 0.098 | |
| ß-NAD | 0.05 | g | 0.05 | - | |
| Coenzyme A | 0.05 | g | 0.05 | 0.065 | |
| FAD | 0.05 | g | 0.05 | 0.064 | |
|
Haemin
(dissolved separately in 10 mM NaOH) |
65.00 | mg | 0.065 | 0.1 | |
| Nicotinamide | 25.00 | mg | 0.025 | 0.205 | |
| Folic acid | 2.50 | mg | 0.003 | 0.006 | |
| Riboflavin | 0.50 | mg | 5.0e-4 | 0.001 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.0 with 1 N NaOH against indicator paper, filter sterilize and store under 100% N2 gas atmosphere. | |||||
Media with this solution
| 843 | HELMUTKOENIGIA MEDIUM |