Loading ...

Solutions

Solution finder
Back to all solutions

Main sol. 25

Internal Solution-ID: S1732

Equipment needed: Tungsten lamp
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.25 g 0.25 -
2 1.50 g 1.5 8.234
3 0.50 g 0.5 -
4 0.50 g 0.5 3.674
5 0.40 g 0.4 1.623
6 0.40 g 0.4 7.478
7 0.40 g 0.4 6.844
8 0.05 g 0.05 0.34
9 Trace element solution SL-6 1.00 ml - -
10 1000.00 ml 998.207 55408.8
11
(0.1% in H2O)
5.00 ml - -
12
(0.002% in H2O)
1.00 ml 8.0e-6 3.3e-5
13
(0.01% in H2O)
1.00 ml - -
14 , add 0.2 ml to each 100 ml media
(10 %)
x - -
Instructions:
  1. Adjust pH to 4.9, then add: Fe(III) citrate solution, Biotin solution, and p-Aminobenzoic acid solution (#11-13)
  2. Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp.
25 RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM