Main sol. 887
Internal Solution-ID: S1836
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 256.00 | mg | 0.256 | 4.381 | |
| KH2PO4 | 1.70 | mg | 0.002 | 0.012 | |
| CaCl2 x 2 H2O | 0.80 | mg | 8.0e-4 | 0.005 | |
| H3BO3 | 10.30 | mg | 0.01 | 0.167 | |
| Na2SO4 | 23.00 | mg | 0.023 | 0.162 | |
| NaNO3 | 0.30 | mg | 3.0e-4 | 0.004 | |
| KCl | 15.00 | mg | 0.015 | 0.201 | |
| FeCl3 x 6 H2O | 0.10 | mg | 1.0e-4 | 3.7e-4 | |
| MnSO4 x 4 H2O | 0.06 | mg | 6.0e-5 | 2.7e-4 | |
| Modified Wolin's mineral solution II | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Sparge medium with 100% N2 gas mixture for 30 min to make anoxic. | |||||
| 2 Adjust pH to 7.0 with H2SO4. | |||||
| 3 Fill 10 ml medium into 100 ml Serum bottles under a stream of H2/CO2 gas and seal with a rubber stopper. | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
| 5 After autoclaving add the following (e.g., from sterile stock solutions) | |||||
|
Na2S2O3 x 5 H2O
(10% in H2O) |
1.00 | g/l | 1 | 4.029 | |
|
NaHCO3
(5 % in H2O) |
1.00 | g/l | 1 | 11.904 | |
| 6 Adjust the gas atmosphere strain specifically. | |||||
Media with this solution
| 887 | OS MEDIUM |