Main sol. 901
Internal Solution-ID: S1853
Equipment needed: Hungate tubes
Filter
Gassing station
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.33 | g | 0.324 | 6.066 | |
| KCl | 0.33 | g | 0.324 | 4.353 | |
| KH2PO4 | 0.33 | g | 0.324 | 2.384 | |
| MgCl2 x 6 H2O | 0.33 | g | 0.324 | 1.596 | |
| CaCl2 x 2 H2O | 0.33 | g | 0.324 | 2.207 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
Sulfur
(powdered) |
10.00 | g | 9.833 | 306.606 | |
| Yeast extract | 3.00 | g | 2.95 | - | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Neutralized sulfide solution 3% (w/v) | 15.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except sulfur, yeast extract, vitamins and sulfide. Adjust pH to 3.5 with H2SO4 and sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials containing the appropriate amount of sulfur. Heat vessels containing medium to 90°C for 1 hour on each of 3 succesive days. Add yeast extract, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The sulfide stock solution (3% w/v) should be neutralized before use (see medium 29). The pH of the complete medium should be at 3.5 - 3.8. | |||||
Media with this solution
| 901 | ACIDOLOBUS ACETICUS MEDIUM |