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Solution B

Internal Solution-ID: S1870

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine pyrophosphate 6 mg 0.133 0.289
NaHCO3 2 g 44.444 529.056
Distilled water 25 ml - -
Volatile fatty acid solution 20 ml - -
Rabbit serum 20 ml - -
1 Sterilize by filtration, add 20 ml rabbit serum, gas with 80% N2 + 20% CO2.
909 TREPONEMA DENTICOLA MEDIUM