DSMZ 909 . TREPONEMA DENTICOLA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 10.00 ml - -
Solution B 0.45 ml - -
1 Add 0.45 ml Sol. B to 10 ml Sol. A. Inoculate and pressurize the tubes with H2 to 0.5 bar.
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Brain heart infusion broth 12.50 g 12.5 -
Trypticase
(BBL 11921)
10.00 g 10 -
Yeast extract 2.50 g 2.5 -
Na-thioglycolate 0.50 g 0.5 4.382
L-Cysteine HCl 1.00 g 1 6.344
L-Asparagine 0.25 g 0.25 1.892
Glucose 2.00 g 2 11.101
Agar 3.00 g 3 8.92
Distilled water 1000.00 ml - -
1 Boil for 5 min, cool to 50°C while gassing with 80% N2 + 20% CO2. Distribute to anaerobic tubes under the same atmosphere and autoclave.
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine pyrophosphate 6 mg 0.133 0.289
NaHCO3 2 g 44.444 529.056
Distilled water 25 ml - -
Volatile fatty acid solution 20 ml - -
Rabbit serum 20 ml - -
1 Sterilize by filtration, add 20 ml rabbit serum, gas with 80% N2 + 20% CO2.
Compound Amount Unit Conc. [g/L] Conc. [mM]
KOH
(0.1 N)
100.0 ml - -
Isobutyric acid 0.5 ml - -
DL-2-Methylbutyric acid 0.5 ml - -
Isovaleric acid 0.5 ml - -
Valeric acid 0.5 ml - -

Metadata

Last modified: 29.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Brain heart infusion broth 0.12 -
Trypticase 0.096 -
Agar 0.029 0.085
Yeast extract 0.024 -
Glucose 0.019 0.106
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain