Main sol. 911
Internal Solution-ID: S1873
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto Middelbrook 7H11 agar (Difco 283810) | 21 | g | 11.024 | - | |
| Glycerol | 5 | ml | - | - | |
| Distilled water | 900 | ml | - | - | |
| Bacto Middelbrook OADC enrichment (Difco 212240): | 100 | ml | - | - | |
| 1 Bacto Middelbrook OADC enrichment (Difco 212240): | |||||
| 2 Sterilize for 10 min by 121°C cool to 50 - 55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco (see below) under aseptic conditions and mix thoroughly. | |||||
| 3 Final pH at 25°C 6.6 ± 0.2. | |||||
Media with this solution
| 911 | MIDDLEBROCK 7H11 AGAR |