DSMZ 911 . MIDDLEBROCK 7H11 AGAR

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto Middelbrook 7H11 agar (Difco 283810) 21 g 11.024 -
Glycerol 5 ml - -
Distilled water 900 ml - -
Bacto Middelbrook OADC enrichment (Difco 212240): 100 ml - -
1 Bacto Middelbrook OADC enrichment (Difco 212240):
2 Sterilize for 10 min by 121°C cool to 50 - 55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco (see below) under aseptic conditions and mix thoroughly.
3 Final pH at 25°C 6.6 ± 0.2.
Compound Amount Unit Conc. [g/L] Conc. [mM]
Pancreatic digest of casein 1.0 g 1.111 -
(NH4)2SO4 0.5 g 0.556 4.204
KH2PO4 1.5 g 1.667 12.247
Na2HPO4 1.5 g 1.667 11.741
Sodium citrate 0.4 g 0.444 1.722
MgSO4 50.0 mg 0.056 0.462
L-Glutamic acid 0.5 g 0.556 3.776
Ferric ammonium citrate 40.0 mg 0.044 -
Pyridoxine 1.0 mg 0.001 0.007
Biotin 0.5 mg 5.6e-4 0.002
Malachite green 1.0 mg 0.001 0.003
Agar
(Bacto )
15.0 g 16.667 49.555
Distilled water 900.0 ml - -
Compound Amount Unit Conc. [g/L] Conc. [mM]
Oleic acid 50 mg 0.5 1.77
Bovine albumin
(albumin fraction V)
5 g 50 -
Dextrose 2 g 20 100.923
Catalase
(beef)
4 mg 0.04 -
NaCl 850 mg 8.5 145.448
Distilled water 100 ml - -

Metadata

Last modified: 25.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.4 - 6.8
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Bovine albumin 5 -
Dextrose 2 10.092
NaCl 0.85 14.545
Agar 0.35 1.041
Oleic acid 0.05 0.177
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Cultivation metadata from 3 strains BacDive

phyla 1 2 strains
oxygen aerobe 1 strain
temperature thermophilic 2 strains