911
911 .
MIDDLEBROCK 7H11 AGAR
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 911 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Bacto Middelbrook 7H11 agar (Difco 283810) | 21 | g | 11.024 | - | |
| Glycerol | 5 | ml | - | - | |
| Distilled water | 900 | ml | - | - | |
| Bacto Middelbrook OADC enrichment (Difco 212240): | 100 | ml | - | - | |
| 1 Bacto Middelbrook OADC enrichment (Difco 212240): | |||||
| 2 Sterilize for 10 min by 121°C cool to 50 - 55°C. Add 100 ml Bacto Middelbrook OADC enrichment Difco (see below) under aseptic conditions and mix thoroughly. | |||||
| 3 Final pH at 25°C 6.6 ± 0.2. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Pancreatic digest of casein | 1.0 | g | 1.111 | - |
| (NH4)2SO4 | 0.5 | g | 0.556 | 4.204 |
| KH2PO4 | 1.5 | g | 1.667 | 12.247 |
| Na2HPO4 | 1.5 | g | 1.667 | 11.741 |
| Sodium citrate | 0.4 | g | 0.444 | 1.722 |
| MgSO4 | 50.0 | mg | 0.056 | 0.462 |
| L-Glutamic acid | 0.5 | g | 0.556 | 3.776 |
| Ferric ammonium citrate | 40.0 | mg | 0.044 | - |
| Pyridoxine | 1.0 | mg | 0.001 | 0.007 |
| Biotin | 0.5 | mg | 5.6e-4 | 0.002 |
| Malachite green | 1.0 | mg | 0.001 | 0.003 |
|
Agar
(Bacto ) |
15.0 | g | 16.667 | 49.555 |
| Distilled water | 900.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Oleic acid | 50 | mg | 0.5 | 1.77 |
|
Bovine albumin
(albumin fraction V) |
5 | g | 50 | - |
| Dextrose | 2 | g | 20 | 100.923 |
|
Catalase
(beef) |
4 | mg | 0.04 | - |
| NaCl | 850 | mg | 8.5 | 145.448 |
| Distilled water | 100 | ml | - | - |
Metadata
| Last modified: | 25.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.4 - 6.8 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Bovine albumin | 5 | - |
| Dextrose | 2 | 10.092 |
| NaCl | 0.85 | 14.545 |
| Agar | 0.35 | 1.041 |
| Oleic acid | 0.05 | 0.177 |
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