Main sol. 928
Internal Solution-ID: S1912
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 22.00 | g | 22 | 207.571 | |
| NaHCO3 | 8.00 | g | 8 | 95.23 | |
| NaCl | 5.00 | g | 5 | 85.558 | |
| KNO3 | 0.50 | g | 0.5 | 4.945 | |
| K2HPO4 | 0.50 | g | 0.5 | 2.871 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Peptone | 1.00 | g | 1 | - | |
| Sodium acetate | 1.60 | g | 1.6 | 19.505 | |
| MgSO4 | 0.06 | g | - | - | |
| Trace elements solution (Pfennig & Lippert,1966) | 2.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
|
Agar
, for solid medium
optional |
20.00 | g | 20 | - | |
| 1 Final pH should be about 10.0 | |||||
| 2 Note: If the medium is solidified with agar prepare the medium given above in 500 ml of distilled water and 20 g of agar in another container with 500 ml distilled water. Autoclave separately and allow to cool to about 50°C before mixing. | |||||
Media with this solution
| 928 | ROSEINATRONOBACTER MEDIUM |