DSMZ 928 . ROSEINATRONOBACTER MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 22.00 g 22 207.571
NaHCO3 8.00 g 8 95.23
NaCl 5.00 g 5 85.558
KNO3 0.50 g 0.5 4.945
K2HPO4 0.50 g 0.5 2.871
Yeast extract 1.00 g 1 -
Peptone 1.00 g 1 -
Sodium acetate 1.60 g 1.6 19.505
MgSO4 0.06 g - -
Trace elements solution (Pfennig & Lippert,1966) 2.00 ml - -
Distilled water 1000.00 ml - -
Agar , for solid medium
optional
20.00 g 20 -
1 Final pH should be about 10.0
2 Note: If the medium is solidified with agar prepare the medium given above in 500 ml of distilled water and 20 g of agar in another container with 500 ml distilled water. Autoclave separately and allow to cool to about 50°C before mixing.
Solution occurs in 28 other media (first defined in Medium 1369).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.20 g 2.2 7.913
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.03 g 0.03 0.485
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.03 g 0.03 0.176
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 pH 3.0-4.0

Metadata

Last modified: 26.05.23
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 10.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 22 207.571
Agar 20 -
NaHCO3 8 95.23
NaCl 5 85.558
Sodium acetate 1.6 19.505
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain