Main sol. 959a
Internal Solution-ID: S1980
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.00 | g | 26 | 444.901 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.5 | 2.459 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| KCl | 0.20 | g | 0.2 | 2.683 | |
|
PIPES
(SIGMA) |
3.40 | g | 3.4 | 11.245 | |
| K2HPO4 | 0.35 | g | 0.35 | 2.009 | |
| KH2PO4 | 0.35 | g | 0.35 | 2.572 | |
| Na-acetate x 3 H2O | 2.72 | g | 2.72 | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 2 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Maltose | 3.50 | g | 3.5 | 10.225 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except maltose and sulfide, adjust pH to 5.8 - 6.0 and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. The pH of the complete medium should be 6.0. | |||||
Media with this solution
| 959a | MARINITOGA LITORALIS MEDIUM |