Main sol. 999
Internal Solution-ID: S2037
Equipment needed: Tungsten lamp
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 1.00 | g | 1 | 7.348 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| NaCl | 100.00 | g | 100 | 1711.16 | |
| MgCl2 x 6 H2O | 2.00 | g | 2 | 9.838 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 After autoclaving 20 min at 121°C, the following sterile solutions are added: | |||||
|
NaHCO3
(20%) |
10.00 | ml | 2 | 23.808 | |
|
Na2S x 9 H2O
(10%) |
7.50 | ml | 0.75 | 3.123 | |
| Vitamins V7 solution (Pfennig et Trüper, 1992) | 1.00 | ml | - | - | |
| Trace element solution SL12 | 1.00 | ml | - | - | |
| 2 The pH is adjusted to pH 7.2-7.4 For better growth of pure cultures, the growth medium should be supplemented with acetate (2 mM final concentration), succinate (1 mM final concentration) and yeast extract (0.5 g/l). Pure cultures should be incubated at 30°C and light intensity of 500 lux from a tungsten lamp (16 h light, 8 h dark). Pure cultures in liquid medium can be stored in 60 ml screw cap bottles at 4°C in the dark. | |||||
Media with this solution
| 999 | HALORHODOSPIRA MEDIUM |