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999. HALORHODOSPIRA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Tungsten lamp
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.98 7.204
2 0.05 g 0.049 0.333
3 0.50 g 0.49 9.164
4 100.00 g 98.039 1677.53
5 2.00 g 1.961 9.645
6 1.00 g 0.98 3.978
7 1000.00 ml 978.634 54322.4
8
(20%)
10.00 ml 4.233 50.393
9
(10%)
7.50 ml - -
10 Vitamins V7 solution (Pfennig et Trüper, 1992) 1.00 ml - -
11 Trace element solution SL12 1.00 ml - -
12 , might need strain adjustment 2.00 mM - -
13 , might need strain adjustment 1.00 mM - -
14 , might need strain adjustment 0.50 g/l - -
Instructions:
  1. After autoclaving 20 min at 121°C, the following sterile solutions are added: #8-11
  2. The pH is adjusted to pH 7.2-7.4 For better growth of pure cultures, the growth medium should be supplemented with acetate (2 mM final concentration), succinate (1 mM final concentration) and yeast extract (0.5 g/l). Pure cultures should be incubated at 30°C and light intensity of 500 lux from a tungsten lamp ( 16 h light, 8 h dark). Pure cultures in liquid medium can be stored in 60 ml screw cap bottles at 4°C in the dark.
Solution occurs in 1 other media (first defined in medium 998 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
10 1000 ml 998.207 55408.8
11 2 mg 0.002 0.008
12 10 mg 0.01 0.073
13 10 mg 0.01 -
14 5 mg 0.005 0.023
15 50 mg 0.05 0.297
16 20 mg 0.02 0.013
17 20 mg 0.02 0.164
Instructions:
  1. Sterilized by filtration.
Solution occurs in 1 other media (first defined in medium 998 )
Equipment needed: Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1000.0 ml 998.207 55408.8
2 300.0 mg 0.3 4.852
3 1.1 g 1.1 3.957
4 190.0 mg 0.19 0.799
5 50.0 mg 0.05 0.309
6 42.0 mg 0.042 0.308
7 24.0 mg 0.024 0.101
8 18.0 mg 0.018 0.074
9 2.0 mg 0.002 0.012
Instructions:
  1. Sterilized by filtration.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Filter, Tungsten lamp

Associated strains

All strains for this medium: 2
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 980.591 54431
NaCl 98.039 1677.53
NaHCO3 4.233 50.393
MgCl2 x 6 H2O 1.961 9.645
KH2PO4 0.98 7.204
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature mesophilic 2 strains

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39. PFENNIG'S MEDIUM I WITH YEAST EXTRACT 94 %
536. THIOCAPSA HALOPHILA MEDIUM 94 %

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