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Main sol. 1025

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Internal Solution-ID: S2083

Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sigma Sea salts 30.0 g 30 -
NH4Cl 0.5 g 0.5 9.348
Distilled water 1000.0 ml - -
50xPYE 20.0 ml - -
Riboflavin
(0.2 mg/ml) 		5.0 ml - -
Glucose
(50 %) 		2.0 ml 1 5.551
1 Autoclave and cool the medium. Aseptically add from sterile stock solutions, 20 ml 50xPYE and 5 ml 0.2 mg/ml riboflavin (filter sterilised), and 2 ml 50% glucose per liter of medium.
1025 MARICAULIS MEDIUM