Main sol. 1058a
Internal Solution-ID: S2137
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 233.00 | g | 233 | 3987 | |
| K2HPO4 | 1.50 | g | 1.5 | 8.612 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.34 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| Na2S2O3 x 5 H2O | 5.00 | g | 5 | 20.146 | |
| NaHCO3 | 2.50 | g | 2.5 | 29.759 | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under air atmosphere in Erlenmeyer flasks to 10% of volume, close with screw caps and autoclave. Add trace elements, calcium chloride, magnesium chloride, thiosulfate, bicarbonate and vitamins from sterile stock solutions and adjust pH of the medium to 7.0 - 7.2, if necessary. Incubate with shaking. | |||||
| 2 Note: Use at least 10% (v/v) as inoculum. | |||||
Media with this solution
| 1058a | THIOHALOSPIRA HALOPHILA MEDIUM |