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Main sol. 1080

Internal Solution-ID: S2186

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
PPLO broth 17 g 17 -
Yeast extract
(pH 7.0, autoclaved, 25%)
100 ml 25 -
Phenol red
(pH 7.0, autoclaved, 1%)
20 ml 0.2 0.564
Distilled water 670 ml - -
Sterile supplement 210 ml - -
1 Add all components except sterile supplement; adjust pH to 7.2 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement.
1080 MYCOPLASMA MEDIUM (CIP MEDIUM 89)