Main sol. 1080
Internal Solution-ID: S2186
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
PPLO broth | 17 | g | 17 | - | |
Yeast extract
(pH 7.0, autoclaved, 25%) |
100 | ml | 25 | - | |
Phenol red
(pH 7.0, autoclaved, 1%) |
20 | ml | 0.2 | 0.564 | |
Distilled water | 670 | ml | - | - | |
Sterile supplement | 210 | ml | - | - | |
1 Add all components except sterile supplement; adjust pH to 7.2 + 0.2. Autoclave at 121°C for 15 minutes; add aseptically sterile supplement. |
1080 | MYCOPLASMA MEDIUM (CIP MEDIUM 89) |