Main sol. 1095
Internal Solution-ID: S2209
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.0 | g | 99.8 | 1707.74 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| MgCl2 | 0.1 | g | 0.1 | 1.048 | |
| NH4Cl | 0.5 | g | 0.499 | 9.329 | |
|
Yeast extract
(OXOID) |
10.0 | g | 9.98 | - | |
|
Tryptone
(BD Bacto) |
10.0 | g | 9.98 | - | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Na2CO3 | 68.0 | g | 67.864 | 640.301 | |
| NaHCO3 | 38.0 | g | 37.924 | 451.44 | |
| L-Cysteine HCl x H2O | 0.7 | g | 0.699 | 3.978 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Sucrose | 5.0 | g | 4.99 | 14.578 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonates, cysteine, sucrose and vitamins), then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add carbonate, hydrogencarbonate and cysteine and adjust pH to 8.5. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sucrose and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. | |||||
Media with this solution
| 1095 | NATRANAEROBIUS MEDIUM |