Main sol. 1101a
Internal Solution-ID: S2218
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 25.00 | g | 24.925 | 426.51 | |
| K2HPO4 | 0.50 | g | 0.499 | 2.862 | |
| Yeast extract | 0.20 | g | 0.199 | - | |
| Na2SO4 | 3.00 | g | 2.991 | 21.058 | |
| NaHCO3 | 1.85 | g | 1.844 | 21.956 | |
| Na2CO3 | 1.20 | g | 1.196 | 11.288 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.49 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na-L-lactate | 2.20 | g | 2.193 | - | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.499 | 2.076 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonates, magnesium chloride, trace elements, lactate, vitamins and sulfide, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then add and dissolve carbonates, adjust pH to 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium chloride, trace elements, lactate, vitamins and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 9.0 - 9.5, if necessary. | |||||
Media with this solution
| 1101a | DESULFONATRONUM ZHILINAE MEDIUM |