Hutners basal salts
Internal Solution-ID: S2294
This solution is used in 4 media and was first defined in medium 1144.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.00 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.70 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.34 | g | 3.34 | 22.719 | |
| (NH4)6Mo7O24 x 4 H2O | 9.25 | mg | 0.009 | - | |
| FeSO4 x 7 H2O | 99.00 | mg | 0.099 | 0.356 | |
| "Metals 44" | 50.00 | ml | - | - | |
| Distilled water | 950.00 | ml | - | - | |
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||