Main sol. 134
Internal Solution-ID: S230
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 27.00 | mg | 0.027 | 0.198 | |
| K2HPO4 | 40.00 | mg | 0.04 | 0.23 | |
| Na2HPO4 x 2 H2O | 40.00 | mg | 0.04 | 0.282 | |
| CaCl2 x 2 H2O | 50.00 | mg | 0.05 | 0.34 | |
| MgSO4 x 7 H2O | 75.00 | mg | 0.075 | 0.304 | |
| FeCl3 x 6 H2O | 5.00 | mg | 0.005 | 0.018 | |
| MnSO4 x H2O | 3.00 | mg | 0.003 | 0.018 | |
| Trace element solution SL-6 | 1.00 | ml | - | - | |
| Glutamic acid | 1.31 | g | 1.31 | 8.904 | |
|
Trypticase soy broth
(without glucose, BBL) |
2.50 | mg | 0.003 | - | |
| Thiamine-HCl x 2 H2O | 0.40 | mg | 4.0e-4 | 0.001 | |
| Vitamin B12 | 0.01 | mg | 1.0e-5 | 7.4e-6 | |
| Glucose | 2.00 | g | 2 | 11.101 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 7.5. The vitamins are sterilized by filtration. The glucose is dissolved in 5 ml of distilled water and sterilized separately by autoclaving. | |||||
Media with this solution
| 134 | HALISCOMENOBACTER MEDIUM |