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Main sol. 1149

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Internal Solution-ID: S2307

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 0.78 g 0.78 4.478
KH2PO4 0.75 g 0.75 5.511
Trace element solution 10.00 ml - -
Vitamin solution 10.00 ml - -
MgCl2 x 6 H2O 0.36 g 0.36 1.771
CaCl2 x 2 H2O 0.10 g 0.1 0.68
NH4Cl 0.53 g 0.53 9.908
Na2SO4 0.14 g 0.14 0.986
NaCl 20.00 g 20 342.231
Yeast extract 2.00 g 2 -
NaNO3 1.70 g 1.7 20.001
Distilled water 1000.00 ml - -
1 Prepare the medium without vitamins, boil and cool under a nitrogen:carbon dioxide (80:20 v/v) atmosphere. Vitamins (filter sterilized and stored under nitrogen) and 100 ml 0.6 M NaHCO3 solution (sterilized and stored under nitrogen) are added from stock solutions.
2 The pH should be pH 6.5.
1149 OCEANITHERMUS MEDIUM