Main sol. 1163a
Internal Solution-ID: S2332
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.674 | 507.762 | |
|
HEPES
(SIGMA) |
4.70 | g | 4.649 | 19.508 | |
| KCl | 0.33 | g | 0.326 | 4.378 | |
| MgCl2 x 2 H2O | 0.90 | g | 0.89 | 6.783 | |
| MgSO4 x 7 H2O | 1.40 | g | 1.385 | 5.618 | |
| NH4Cl | 0.25 | g | 0.247 | 4.623 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.138 | 0.942 | |
| KH2PO4 | 0.45 | g | 0.445 | 3.271 | |
| Yeast extract | 5.00 | g | 4.946 | - | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | 2.816 | |
| D-Glucose | 5.00 | g | 4.946 | 27.451 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine, maltose and vitamins. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add and dissolve cysteine and adjust pH to 7.5 with NaOH. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose and vitamins after autoclaving from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. | |||||
Media with this solution
| 1163a | OCEANOTOGA MEDIUM |