Main sol. 1176
Internal Solution-ID: S2348
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 120.00 | g | 120 | 2053.39 | |
| K2HPO4 | 3.00 | g | 3 | 17.224 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| HEPES | 2.38 | g | 2.38 | 9.987 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 After sterilization add from sterile stock solutions add: | |||||
|
MgSO4 x 7 H2O
(1M) |
2.00 | ml/l | 0.493 | 2 | |
|
Sodium acetate
(2M) |
10.00 | ml/l | 1.641 | 20 | |
|
Yeast extract
(10%) |
2.00 | ml/l | 0.2 | - | |
| 2 Adjust pH to 7.5. Dispense the medium into sealed bottles and autoclave. | |||||
Media with this solution
| 1176 | HALOVIBRIO MEDIUM |