Main sol. 1204
Internal Solution-ID: S2404
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 1.00 | g | 1 | 17.112 | |
| KCl | 0.50 | g | 0.5 | 6.707 | |
| NH4Cl | 0.30 | g | 0.3 | 5.609 | |
| KH2PO4 | 0.20 | g | 0.2 | 1.47 | |
| Na2SO4 | 0.15 | g | 0.15 | 1.056 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| Yeast extract | 4.00 | g | 4 | - | |
| Casein peptone | 2.00 | g | 2 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.00 | g | 1 | 9.435 | |
| Maltose monohydrate | 2.00 | g | 2 | 5.551 | |
|
DL-Dithiothreitol
(DTT) |
0.80 | g | 0.8 | 5.185 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, maltose and DTT, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 minutes. Add maltose and DTT from sterile anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration and carbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Check pH of complete medium and adjust to 7.4, if necessary. | |||||
Media with this solution
| 1204 | SPHAEROCHAETA MEDIUM |