Amorphous Fe(OH)3
Internal Solution-ID: S2411
This solution is used in 3 media and was first defined in medium 1210.
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| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
FeCl3 x 6 H2O
(60.00 g/l) |
320 | ml | - | - | |
|
NaOH
, adjust if required
(10% w/v) |
80 | ml | 20 | 500.038 | |
| 1 Slowly titrate 320 ml of a FeCl3 x 6 H2O stock solution (60.00 g/l) with 10% (w/v) NaOH to pH 8.0-8.5 under agitation (use magnetic stirrer). Total amount of added NaOH approx. 80 - 100 ml. The precipitated Fe(OH)3 should be stored at room temperature overnight with surface covered with water. Thereafter, centrifuge at 2000 rpm for 5 min and discard the supernatant. Wash several times with distilled water. Resuspend the pellet in medium as described above. For storage autoclave under 100% N2 atmosphere. | |||||