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Amorphous Fe(OH)3

Internal Solution-ID: S2411

This solution is used in 3 mediaand was first defined in medium 1210.

Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O
(60.00 g/l)
320 ml - -
NaOH , adjust if required
(10% w/v)
80 ml 20 500.038
1 Slowly titrate 320 ml of a FeCl3 x 6 H2O stock solution (60.00 g/l) with 10% (w/v) NaOH to pH 8.0-8.5 under agitation (use magnetic stirrer). Total amount of added NaOH approx. 80 - 100 ml. The precipitated Fe(OH)3 should be stored at room temperature overnight with surface covered with water. Thereafter, centrifuge at 2000 rpm for 5 min and discard the supernatant. Wash several times with distilled water. Resuspend the pellet in medium as described above. For storage autoclave under 100% N2 atmosphere.
1210b PYRODICTIUM DELANEYI MEDIUM
1210 GEOGLOBUS MEDIUM
1277b CALDIMICROBIUM THIODISMUTANS MEDIUM