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1210. GEOGLOBUS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 18.00 g 17.946 307.074
2 4.00 g 3.988 19.616
3 0.33 g 0.329 4.413
4 0.33 g 0.329 2.238
5 0.50 g 0.499 3.773
6 Trace element solution SL-10 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 1.00 g 0.997 9.407
9 1.50 g 1.496 18.231
10 0.33 g 0.329 2.418
11 0.20 g 0.199 -
12 Vitamin solution 1.00 ml - -
13 Amorphous Fe(OH)3 200.00 ml - -
14 800.00 ml 796.177 44194.5
Instructions:
  1. Dissolve ingredients except carbonate, acetate, hydrogenphosphate, yeast extract, vitamins and ferric iron hydroxide sludge. Suspend pellet of ferric iron hydroxide in medium and sparge with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Thereafter, dispense suspension under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave for 30 min. Add acetate, hydrogenphosphate, yeast extract and vitamins (sterilized by filtration) from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. The pH of the complete medium should be at 6.5 - 6.8.
Solution occurs in 2 other media (first defined in medium 1210 )
Equipment needed: Centrifuge Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(60.00 g/l)
320 - -
2
(10% w/v)
80 ml 100 2500.18
3 x - -
Instructions:
  1. Slowly titrate 320 ml of a FeCl3 x 6 H2O stock solution (60.00 g/l) with 10%(w/v) NaOH to pH 8.0-8.5 under agitation (use magnetic stirrer). Total amount of added NaOH approx. 80 - 100 ml. The precipitated Fe(OH)3 should be stored at room temperature overnight with surface covered with water. Thereafter, centrifuge at 2000 rpm for 5 min and discard the supernatant. Wash several times with distilled water. Resuspend the pellet in medium as described above. For storage autoclave under 100% N2 atmosphere.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: 6.6
Equipment needed: Autoclave, Centrifuge, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 20 % CO2
  • 80 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 799.153 44359.7
NaOH 19.941 498.553
NaCl 17.946 307.074
MgCl2 x 6 H2O 3.988 19.616
Sodium acetate 1.496 18.231
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature hyperthermophilic 1 strain

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