Main sol. 1218
Internal Solution-ID: S2424
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.4 | g | 0.4 | 7.478 | |
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| Na2SO4 | 0.5 | g | 0.5 | 3.52 | |
| NaNO3 | 0.4 | g | 0.4 | 4.706 | |
| NaCl | 40.0 | g | 40 | 684.463 | |
| KCl | 0.5 | g | 0.5 | 6.707 | |
| NaHCO3 | 10.0 | g | 10 | 119.038 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| Na2S2O3 x 5 H2O | 1.0 | g | 1 | 4.029 | |
| Vitamin B12 | 10.0 | µg | 1.0e-5 | 7.4e-6 | |
| Sodium pyruvate | 1.0 | g | 1 | 9.088 | |
| Sodium acetate | 1.0 | g | 1 | 12.191 | |
| Peptone | 1.0 | g | 1 | - | |
| Yeast extract | 1.0 | g | 1 | - | |
| Trace element solution SL10 | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave the NaHCO3 and Na2CO3 separately and add to the medium. Add the vitamin B12 solution from a filter sterilised stock solution to the autoclaved, cooled medium. Final is pH 9.0 - 9.5 | |||||
Media with this solution
| 1218 | ROSEINATRONOBACTER MONICUS MEDIUM |