MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 1222

Download this solution as

CSV JSON PDF

Internal Solution-ID: S2429

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.5 3.674
NaCl 20.00 g 20 342.231
NH4Cl 0.60 g 0.6 11.217
CaCl2 x 2 H2O 0.15 g 0.15 1.02
MgSO4 x 7 H2O 1.00 g 1 4.057
Sorbitol OR Na pyruvate 3.00 g 3 16.468
Trace element solution SL-8 1.00 ml - -
Vitamin B12
(20mg/l) 		1.00 ml - -
Distilled water 1000.00 ml - -
1 Prepare the medium under a nitrogen atmosphere, dispense into tubes or bottles fitted with rubber stoppers so that they are half full. Add the vitamin B12 from a filter-sterilised stock solution. Either sorbitol or sodium pyruvate may serve as the carbon source. The final pH of the medium should be 6.8.
1222 RHODOVULUM MARINUM MEDIUM