Main sol. 1224
Internal Solution-ID: S2432
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 50.0 | g | 50 | 855.578 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| NaHCO3 | 15.0 | g | 15 | 178.557 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| K2HPO4 | 0.5 | g | 0.5 | 2.871 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
| Na2S2O3 x 5 H2O | 0.5 | g | 0.5 | 2.015 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Na acetate | 0.5 | g | 0.5 | 6.095 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Final pH 9.0 - 9.5 | |||||
| 2 Prepare and boil the medium in the absence of Na2S x 9 H2O, NaHCO3, Vitamin B12, CaCl2 x 2 H2O, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilised) and Na2S2O3 x 5 H2O from sterile stock solutions. | |||||
Media with this solution
| 1224 | ECTOTHIORHODOSPIRA VARIABILIS MEDIUM |