Main sol. 1232
Internal Solution-ID: S2454
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.50 | g | 0.495 | 9.246 | |
| MgSO4 x 7 H2O | 1.90 | g | 1.879 | 7.625 | |
| MgCl2 x 6 H2O | 1.40 | g | 1.385 | 6.811 | |
| K2HPO4 | 1.60 | g | 1.583 | 9.086 | |
| Na2HPO4 x 2 H2O | 1.00 | g | 0.989 | 6.968 | |
| NaCl | 26.00 | g | 25.717 | 440.06 | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
|
Yeast extract
(OXOID) |
2.00 | g | 1.978 | - | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 1.978 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
|
Starch
(soluble) |
5.00 | g | 4.946 | - | |
| CaCl2 x 2 H2O | 0.56 | g | 0.554 | 3.768 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.30 | g | 0.297 | 1.689 | |
| Na2S x 9 H2O | 0.30 | g | 0.297 | 1.235 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except starch, calcium chloride, vitamins and reducing agents) and adjust pH to 6.5 - 6.8. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials (to 30% of volume) and autoclave. Add starch, calcium chloride, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100 N2 gas. Adjust pH of complete medium to 6.8, if necessary. | |||||
Media with this solution
| 1232 | THERMOTOGA TF(D) MEDIUM |