Main sol. 1264
Internal Solution-ID: S2499
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.0 | g | 30 | 513.347 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| CaCl2 x 2 H2O | 0.3 | g | 0.3 | 2.041 | |
| MgCl2 x 2 H2O | 3.0 | g | 3 | 22.859 | |
|
Starch
(soluble) |
5.0 | g | 5 | - | |
| Yeast extract | 1.0 | g | 1 | - | |
|
Peptone
(Difco) |
5.0 | g | 5 | - | |
|
Phosphate buffer
(10%, pH 7.4) |
10.0 | ml | 1 | - | |
| Trace elements (Pfennig) | 1.0 | ml | - | - | |
| Resazurin | 0.5 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Boil medium and cool under N2 stream on ice. Check pH (7.4). Autoclave aliquots and add the following sterile solutions (per 10 ml): | |||||
|
Na2S
(3%) |
0.1 | ml | 0.003 | 0.038 | |
|
Vitamine B12
(1 mg/10 ml) |
50.0 | µl | - | - | |
Media with this solution
| 1264 | SPIROCHAETA SP. MEDIUM |