Main sol. 1277a
Internal Solution-ID: S2556
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgCl2 x 6 H2O | 0.2 | g | 0.199 | 0.981 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.678 | |
| (NH4)2SO4 | 0.1 | g | 0.1 | 0.755 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.733 | |
| KCl | 0.1 | g | 0.1 | 1.337 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Na2CO3 | 1.0 | g | 0.997 | 9.407 | |
| Na2S2O3 x 5 H2O | 2.5 | g | 2.493 | 10.043 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, thiosulfate and vitamins) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under air atmosphere into Hungate-type tubes or serum vials to 30% of volume, close with butyl rubber septa and autoclave. Add carbonate, thiosulfate and vitamins from stock solutions sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2. Incubate without shaking. | |||||
Media with this solution
| 1277a | SULFURIFUSTIS MEDIUM |