DSMZ 1277a . SULFURIFUSTIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 0.2 g 0.2 0.984
CaCl2 x 2 H2O 0.1 g 0.1 0.68
(NH4)2SO4 0.1 g 0.1 0.757
KH2PO4 0.1 g 0.1 0.735
KCl 0.1 g 0.1 1.341
Trace element solution SL-10 1.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Na2CO3 1.0 g 1 9.435
Na2S2O3 x 5 H2O 2.5 g 2.5 10.073
Wolin's vitamin solution 10.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, thiosulfate and vitamins) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under air atmosphere into Hungate-type tubes or serum vials to 30% of volume, close with butyl rubber septa and autoclave. Add carbonate, thiosulfate and vitamins from stock solutions sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2. Incubate without shaking.
Solution occurs in 263 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%)
10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
Solution occurs in 174 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -
Solution occurs in 357 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium D-(+)-pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
(DL)-alpha-Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 03.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0 - 7.2
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2.5 10.073
Na2CO3 1 9.435
MgCl2 x 6 H2O 0.2 0.984
KCl 0.1 1.341
(NH4)2SO4 0.1 0.757
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Cultivation metadata from 4 strains BacDive

pH 8 - 10 2 strains
pH 6 - 8 2 strains
phyla 1 4 strains
nutrition autotroph 2 strains
nutrition chemolithoautotroph 1 strain
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