Main sol. 1277b
Internal Solution-ID: S2557
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| (NH4)2SO4 | 0.1 | g | 0.1 | 0.757 | |
| KH2PO4 | 0.1 | g | 0.1 | 0.735 | |
| KCl | 0.1 | g | 0.1 | 1.341 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
| Amorphous Fe(OH)3 | 45.0 | ml | - | - | |
| Na2CO3 | 2.5 | g | 2.5 | 23.588 | |
| Na2S2O5 | 0.9 | g | 0.9 | 4.734 | |
| Seven vitamins solution | 1.0 | ml | - | - | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, pyrosulfite and vitamins), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into Hungate-type tubes or serum vials and autoclave. Add pyrosulfite from a 10% (w/v) stock solution adjusted to pH 7.5-8.0 with NaOH, made anoxic with 100% N2 gas and sterilized by filtration. Vitamins are added from a stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration and carbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 7.5 - 8.0. Incubate without shaking. | |||||
Media with this solution
| 1277b | CALDIMICROBIUM THIODISMUTANS MEDIUM |