Main sol. 1291
Internal Solution-ID: S2588
Equipment needed: Hungate tubes
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Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Gellan Gum
(Phytagel) |
0.50 | g | 0.5 | - | |
| (NH4)2SO4 | 4.00 | g | 4 | 30.271 | |
| K2HPO4 | 0.45 | g | 0.45 | 2.584 | |
| CaCl2 x 2 H2O | 0.15 | ml | - | - | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
| MgCl2 x 6 H2O | 1.00 | g | 1 | 4.919 | |
| Yeast extract | 0.01 | g | 0.01 | - | |
| Seven vitamins solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Add gellan gum and boil to dissolve. Then add the remaining ingredients (except magnesium chloride, yeast extract and vitamins), adjust the pH value to 5.0 to 5.3 and fill the medium under an air atmosphere into tubes of the Hungate type or similar vials up to 30% their volume. After autoclaving add magnesium chloride, yeast extract and vitamins from steril stock solutions. Vitamins should be sterilized by filtration. | |||||
| 2 Note: Incubation with shaking may improve cell yield. | |||||
Media with this solution
| 1291 | CHTHONOMONAS MEDIUM |