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DSMZ

DSMZ 1291 . CHTHONOMONAS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1291 #

ml
Equipment needed: Hungate tubes Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Gellan Gum
(Phytagel) 		0.50 g 0.5 -
(NH4)2SO4 4.00 g 4 30.271
K2HPO4 0.45 g 0.45 2.584
CaCl2 x 2 H2O 0.15 ml - -
Trace element solution SL-11 1.00 ml - -
MgCl2 x 6 H2O 1.00 g 1 4.919
Yeast extract 0.01 g 0.01 -
Seven vitamins solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Add gellan gum and boil to dissolve. Then add the remaining ingredients (except magnesium chloride, yeast extract and vitamins), adjust the pH value to 5.0 to 5.3 and fill the medium under an air atmosphere into tubes of the Hungate type or similar vials up to 30% their volume. After autoclaving add magnesium chloride, yeast extract and vitamins from steril stock solutions. Vitamins should be sterilized by filtration.
2 Note: Incubation with shaking may improve cell yield.

Trace element solution SL-11 #

1000
Solution occurs in 22 other media (first defined in Medium 722).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA x 2 H2O 5.2 g 5.2 15.467
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 1000.0 ml - -
1 Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.

Seven vitamins solution #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 03.05.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 5.0 - 5.3
Equipment needed: Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
(NH4)2SO4 4 30.271
MgCl2 x 6 H2O 1 4.919
Gellan Gum 0.5 -
K2HPO4 0.45 2.584
Yeast extract 0.01 -
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
temperature thermophilic 1 strain