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1291. CHTHONOMONAS MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Filter
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 506 ml - -
2 Solution B 500 ml - -
3 Solution C 10 ml - -
Instructions:
  1. Dissolve ingredients of solution A and adjust pH to 6.5. Autoclave solutions A and B separately, let cool down to approx. 70°C and combine both solutions while mixing. To the combined solution add solution C (sterilized by filtration) under aseptic conditions and pour plates by using 20 ml medium per petri dish.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 4.00 g 7.905 59.824
2 0.45 g 0.889 5.106
3 0.60 g 1.186 4.811
4
(0.1% w/v)
5.00 ml 0.01 0.067
5 Trace element solution SL-11 1.00 ml - -
6 500.00 ml 986.371 54751.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1
(Phytagel or Gelrite)
15 g 30 -
2 1 g 2 9.838
3 500 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.01 g 1 -
2 Seven vitamins solution 1.00 ml - -
3 9.00 ml 898.386 49868
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 86 other media (first defined in medium 503 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 mg 0.1 0.063
2 80 mg 0.08 0.583
3 20 mg 0.02 0.082
4 200 mg 0.2 1.625
5 100 mg 0.1 0.21
6 300 mg 0.3 1.459
7 200 mg 0.2 0.536
8 1000 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 993.295 55136.1
Gellan Gum 14.764 -
(NH4)2SO4 3.937 29.794
MgCl2 x 6 H2O 0.984 4.841
MgSO4 x 7 H2O 0.591 2.396
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
phyla 1 1 strain
oxygen aerobe 1 strain
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