Main sol. 1297
Internal Solution-ID: S2595
Equipment needed: Autoclave
Water bath
Filter
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaHCO3 | 25.00 | g | 24.925 | 296.704 | |
| Na2CO3 | 140.00 | g | 139.581 | 1316.95 | |
| NaCl | 15.00 | g | 14.955 | 255.906 | |
| K2HPO4 | 1.00 | g | 0.997 | 5.724 | |
| NH4Cl | 0.20 | g | 0.199 | 3.728 | |
| MgCl2 x 6 H2O | 0.20 | g | 0.199 | 0.981 | |
| Yeast extract | 0.20 | g | 0.199 | - | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Glycerol | 2.00 | g | 1.994 | 21.653 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
|
Sulfur
(powdered) |
2.00 | g | 1.994 | 62.177 | |
| Na2S x 9 H2O | 0.25 | g | 0.249 | 1.038 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve hydrogen carbonate, carbonate, sodium chloride, and hydrogen phosphate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, yeast extract, trace elements, glycerol, vitamins, and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days (see medium 35) and added aseptically to the sterile medium as powder while retaining anoxic conditions. Adjust the pH of the complete medium to 9.8 - 10.0, if necessary. | |||||
Media with this solution
| 1297 | NATRONIELLA SULFIDIGENA MEDIUM |