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1297. NATRONIELLA SULFIDIGENA MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 25.00 g 24.704 294.067
2 140.00 g 138.34 1305.24
3 15.00 g 14.822 253.619
4 1.00 g 0.988 5.673
5 0.20 g 0.198 3.695
6 0.20 g 0.198 0.972
7 0.20 g 0.198 -
8 Trace elements solution (Pfennig & Lippert,1966) 1.00 ml - -
9 Selenite-tungstate solution 1.00 ml - -
10 Vitamin solution 10.00 ml - -
11 2.00 g 1.976 21.46
12
(powdered)
2.00 g 1.976 61.632
13 0.25 g 0.247 1.029
14 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve hydrogencarbonate, carbonate, sodium chloride and hydrogenphosphate, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Dispense solution under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ammonium chloride, magnesium chloride, yeast extract, trace elements, vitamins, glycerol and sulfide from sterile anoxic stock solution prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days and added aseptically to the sterile medium as powder while retaining anoxic conditions. Adjust pH of complete medium to 9.8 - 10.0, if necessary.
Solution occurs in 23 other media (first defined in medium 1369 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.00 g 5 17.228
2 2.20 g 2.2 7.913
3 0.10 g 0.1 0.348
4 0.03 g 0.03 0.152
5 0.03 g 0.03 0.485
6 0.20 g 0.2 0.841
7 0.03 g 0.03 0.176
8 0.03 g 0.03 0.126
9 0.03 g 0.03 0.124
10 1000.00 ml 998.207 55408.8
Instructions:
  1. pH 3.0-4.0
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.9
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Na2CO3 138.34 1305.24
NaHCO3 24.704 294.067
NaCl 14.822 253.619
Sulfur 1.976 61.632
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain

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