Neutralized sulfide solution (B)
Internal Solution-ID: S2701
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2S x 9 H2O | 1.5 | g | 15 | 62.453 | |
| Distilled water | 100.0 | ml | - | - | |
| 1 The sulfide solution is prepared in a vessel sealed with a butyl rubber. | |||||
| 2 Autoclave at 121°C for 15 min under a N2 atmosphere. After cooling to room temperature the pH is adjusted to about 7.3 by adding of sterile 2N H2SO4 drop-wise with a syringe without opening the bottle. The vessel should be shaken continuously to avoid precipitation of elemental sulfur. The final solution should be transparent yellow. | |||||
Media with this solution
| 1344 | LYU03 Medium |